Sr. Research Associate, CAR-T
Company: Theragent, Inc.
Posted on: September 21, 2022
What We Do
Theragent is a privately-owned Contract Development and
Manufacturing Organization (CDMO) focused on novel Cell and Gene
Therapy medicines to meet unmet patient needs. We are a small,
biotech startup headquartered in Arcadia, CA. Our state-of-the-art
facility provide high-quality, reliable, and value-added solutions
to our clientele for challenging pre-clinical and clinical trial
projects. Our innovative cGMP facility offers comprehensive
end-to-end manufacturing and testing capabilities for clinical and
Take the next step in your career and join Theragent today to
change patient lives through the products we help develop and
manufacture. Here, you will contribute your talents in a purposeful
way that impact the lives of patients around the world. You will
develop as an individual by working directly with a team of
seasoned experts and rising stars.
Our values of Innovation, Transparency, Accountability, Integrity,
Diversity, Humility to name a few are embedded in everything we do.
No matter your role may be, Theragent strives to ensure all our
teammates are empowered and given the necessary tools to lead us in
our relentless pursuit to extending patient lives.
To Manufacture and Deliver high-quality, reliable, and value-added
Therapeutic Solutions for Biopharma clients and patients.
Job Summary: -
Theragent is looking for an enthusiastic Researcher with either
academic or industry experience to assist with genetic engineering
of lentiviral vectors expressing antibodies and CAR and in vitro
assays. The candidate will focus on the Discovery of Antibody based
biologics and T/NK cell products as well as assist with planning
and execution of experiments, in support of developing analytical
methods for releasing and characterizing T cell therapy products.
Candidates with prior expertise in phage display library
construction, screening and antibody engineering will be given
The successful candidate will demonstrate a strong sense of
responsibility, open communication, desire to learn new skills,
ability to meticulously perform instructed tasks and maintain lab
records. S/he will perform bench work to support research projects
in coordination with our scientific director. We are looking for
enthusiastic individuals with a flexibility to adapt to startup
- Perform flow cytometry-based characterization of CAR-T cells
and functional assays to measure T cell activation, proliferation
(CFSE) and cytotoxicity.
- Immunophenotype CAR-T products under different conditions to
evaluate exhaustion and persistence using flow cytometry.
- Proficiently use flow cytometry analysis software (Flowjo, FCS
express, etc..) and precisely document data using Excel, GraphPad
Prism, Powerpoint, Photoshop, etc---
- Process blood products for or immune cell subsets (T and NK)
using magnetic bead-based technologies (MACs column or EasySep) and
optimize culture conditions for expansion and banking.
- Execute lentiviral/adenoviral production, concentration and
quantitation assays (Flow cytometry, ELISA and qPCR).
- Assist generation of novel lentiviral CAR constructs using
molecular biology techniques (PCR, Gibson cloning).
- Support development of engineered mammalian cell lines and
validation. Assist in creating and revising Standard Operating
Procedures (SOP) for production and validation of viral vectors and
- Routinely passage and maintain cell banks (tumor cell lines and
- Maintain and trouble shoot scientific instruments to ensure
- Precisely plan and execute lab work, analyze data and keep
records up to date and demonstrate ability to track them with
Required Experience/ Education:
- Bachelor's Degree, Master's Degree, or PhD (preferred) in the
field of Immunology, Virology, or related areas. Knowledge of T
cell immunology with insight into T cell effector function,
including T cell differentiation, T cell exhaustion and
persistence, and stimulatory/inhibitory regulatory mechanisms
- Experience with lentiviral transduction and other gene therapy
techniques (e.g. CRISPR).
- Experience in assessing T cell functionality using
multi-parameter flow cytometry for phenotypic characterization, T
cell activation and proliferation analysis.
- Must possess good working knowledge of Word, Excel, Power
Point, GraphPad Prism, and Flow cytometry analysis software
(Flowjo, FCS express)
- Professional Knowledge Problem Solving Team Player / Building
Relationships Multi-tasking Customer Focus Action and detail
oriented Active Listening
Decisive Decision Making
Physical / Mental Demands:
- Occasional stooping, bending, stretching, pushing, pulling,
reaching and/or lifting up to 40lbs. Ability to sit, stand, walk,
and move within workspace for extended periods. Ability to perform
repetitive tasks including hand to finger manipulations, grasping,
pushing, and pulling.
- Primarily office environment but will be required to enter
laboratory or GMP process areas donning proper gowning / lab coats
or PPE such as safety glasses and shoes. Ability to work safely and
effectively when working alone or working with others.
* This description outlines the basic responsibilities and
requirements for the position noted. This is not a comprehensive
listing of all job duties of position. Duties, responsibilities and
activities may change at any time with or without notice.
*Theragent Inc is an equal opportunity employer and prohibits
unlawful discrimination based on race, color, religion, gender,
sexual orientation, gender identity or expression, national origin
or ancestry, age, disability, marital status, and veteran status.
All qualified applicants will receive consideration for
Theragent, Inc. requires employees to be vaccinated against
COVID-19. To the extent permitted by applicable law, candidate must
be able to provide proof of full vaccination or receipt of the
first dose of a two-dose COVID-19 vaccine, or request an exemption
due to a medical reason, a sincerely held religious belief of
practice, or pregnancy, as of the first day of work, to be
considered for a U.S.-based job.
Keywords: Theragent, Inc., Arcadia , Sr. Research Associate, CAR-T, Other , Arcadia, California
Didn't find what you're looking for? Search again!