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Senior Research Associate - Cell Biology

Company: Actalent
Location: Arcadia
Posted on: September 16, 2022

Job Description:

Description:

  • Perform flow cytometry-based characterization of CAR-T cells and functional assays to measure T cell activation, proliferation (CFSE) and cytotoxicity.
  • Immunophenotype CAR-T products under different conditions to evaluate exhaustion and persistence using flow cytometry.
  • Proficiently use flow cytometry analysis software (Flowjo, FCS express, etc..) and precisely document data using Excel, GraphPad Prism, Powerpoint, Photoshop, etc
  • Process blood products for or immune cell subsets (T and NK) using magnetic bead-based technologies (MACs column or EasySep) and optimize culture conditions for expansion and banking.
  • Execute lentiviral/adenoviral production, concentration and quantitation assays (Flow cytometry, ELISA and qPCR).
  • Assist generation of novel lentiviral CAR constructs using molecular biology techniques (PCR, Gibson cloning).
  • Support development of engineered mammalian cell lines and validation. Assist in creating and revising Standard Operating Procedures (SOP) for production and validation of viral vectors and CAR-T products.
  • Routinely passage and maintain cell banks (tumor cell lines and T cells).
  • Maintain and trouble shoot scientific instruments to ensure their functionality.
  • Precisely plan and execute lab work, analyze data and keep records up to date and demonstrate ability to track them with clarity.Skills & Qualifications:
    • Antibody experience
    • Molecular biology experience
    • Dna manipulation/joining pieces of dna together (dna cloning)
    • Cell engineering
    • Experience in phage display library construction, antibody engineering or affinity maturation of antibodies . 3-4 years
    • Cell based assayy & mammalian cell culturing Bachelors + 5 yrs of experience OR Masters + 2 years experience
      • Experience: mammalian cell culture, antibody & phage display technology, assays, dna, pcr About Actalent: Actalent connects passion with purpose. Our scalable talent solutions and services capabilities drive value and results and provide the expertise to help our customers achieve more. Every day, our experts around the globe are making an impact. We're supporting critical initiatives in engineering and sciences that advance how companies serve the world. Actalent promotes consultant care and engagement through experiences that enable continuous development. Our people are the difference. Actalent is an operating company of Allegis Group, the global leader in talent solutions. The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.

Keywords: Actalent, Arcadia , Senior Research Associate - Cell Biology, Other , Arcadia, California

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